Results for "University of Teramo, Department of Comparative Biomedical Sciences"

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Trophoblastic exchange in ruminant embryos

We therefore set up two pilot experiments on sheep and bovine embryos. In the first, we transferred ICM and trophoblastic vesicles between in vitro fertilized sheep blastocysts (Photo 1) . Notwithstanding the aggressive manipulation procedures, 43% of the reconstructed embryos that were transferred ...

Endangered animals, embryo, ICM exchange.

Assessing the functionality of lyophilized chromosomes

In this R&D process we have demonstrated that thanks to the addiction of trehalose, lyophilised somatic cells stored for 3 years at room temperature were able to direct embryonic development following injection into enucleated oocytes. ...

Somatic cells, lyophilization, nuclear transfer, embryo

Inter Specific Somatic Cells Nuclear Transfer (ISCNT)

In this thematic area, as an internationally qualified scientist working in the field of ISCNT I have been asked by the Editor of Theriogenology, the most know international scientific journal on Reproduction, to prepare a review on the open problems of ISCNT. Therefore, in association with Prof. Ja...

Endangered Species, Inter Specific Somatic Cell Nuclear transfer

Improving the efficiency of somatic cell nuclear transfer (SCNT).

In this section I will provide data related only to BRDT transfection and nuclear transfer, cause the PM1 &2 . Primary cultures of sheep fibroblast (previously checked for normal karyotype) have been transfected with the BrDT-GFP plasmid (Lipofectamin, ™ In vitrogen, and Optimel, Gibco), cultured fo...

Sperm specific proteins, somatic cells transfection, nuclear transfer, sheep.

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